1) This is normal for old subfloors. The gaps are not a problem. You should cover the subfloor planks with 15 lb. asphalt saturated roofing felt paper. If the old section did not have it, you still should put it in the area you are replacing. Be VERY careful driving screws into those old dry planks. Odds are, they will crack and split. If you must use screws, pre-drill pilot holes first.
2) If you can carefully remove the subfloor planking, fur up the joist a little, then re-install the planking without damaging it, then I say no reason why you cannot re-use it.
3) Do not bother trying to fill holes or gaps. Those are normal with these types of subfloors. You may experience some noise or squeaks with that subfloor. If you can get the noises out, great. If not, then live with it. It is an old home just doing what old homes do.
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