The old house I live in and the old house my son in laws, (both of them) there is only a single floor) no sub floor and I have seen a number with just the tongue and grove fir flooring as the only floor, no sub flooring, my house was built in about 1905, my one SILs house was built in about the same time, and the second SIL house was build in 1928.
A reasonably common practice in our area, for he time (home steading in Colorado in the early 1900's),
when I have worked on them and a new floor is wanted, I usually lay a Plywood underlayment, to level and reinforce the existing floor, if needed, and some depends on the type of floor that is being laid,
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