Laying a new floor over OSB is not a problem. Staples will hold better than cleats.Go across the floor joists. Nailing into the joists may help, but is not a requirement for a good floor. If you want a tighter, more secure floor then use narrow strips which will give you more fasteners per square foot. Adding another layer of plywood will add the possibility that this material will not be fastened securely to the sub-floor, and you will get less penetration into the OSB with your florin g fasteners. Essentially, you will be re-fastening the 3/8th to the OSB as the fasteners go through the plywood, unless you glue the plywood to the OSB with full coverage. This is a lot of work that I think is unnecessary. Try fastening a couple of rows of flooring and then try to pull it up with a 6 inch nailing schedule instead of 8-10 inches. Fasteners are inexpensive, use more if you think that they don't hold good enough, or as well as into plywood.
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